In LD50 testing, mortality is tracked over how many days?

• A. 7 days
• B. 14 days
• C. 21 days
• D. 28 days

Answer: (B)

The main idea is that LD50 is about acute lethality after a single exposure, measured within a standard observation window. That window is 14 days because most deaths from acute toxic effects occur within two weeks of dosing, so this period captures the majority of acute fatalities. If you only watched for 7 days, you’d miss late-onset deaths that still relate to the dose. If you watched for 21 or 28 days, you’d start including longer-term health effects and recovery processes that aren’t part of acute lethality, making the LD50 endpoint less about immediate toxicity. Regulatory testing and classic LD50 methods use a 14-day observation to define the dose that kills 50% of animals within that timeframe.